Epilife medium with 60 μm calcium
WebJan 1, 2024 · Neonatal human epidermal keratinocytes (NHEK) (Thermo Fisher Scientific, C0015C) were cultured in Epilife Medium with 60 μM calcium (Thermo Fisher Scientific, MEPI500CA) supplemented with human keratinocyte growth supplement (Thermo Fisher Scientific, S0015) and 0.5% antibiotic/antimycotic solution (antibiotic antimycotic solution, … WebSpecifications Description A sterile, liquid medium prepared with 60μM calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes and human corneal …
Epilife medium with 60 μm calcium
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WebEpiLife™ Medium, with 60 µM calcium. Brand: Gibco™ MEPI500CA. 54.10 GBP valid until 2024-03-27. Use promo code "20585" to get your promotional price. Code : 50. … WebEpiLife is a sterile, liquid medium prepared with 60 µM calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. … EpiLife is a sterile, liquid medium prepared with 60 µM calcium chloride for the long … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes
WebMar 14, 2024 · Primary murine epidermal keratinocytes were obtained, as described previously (Li et al., 2024) and cultured in EpiLife™ medium with 60 μM calcium. The medium was supplemented with 1% (v/v) penicillin/streptomycin (10,000 U and 10,000 μg/ml), and the cells were incubated in a humidified atmosphere with 5% CO 2 at 37°C. … WebAug 1, 2024 · To study the consequences of GO treatment on proliferative KCs, we first seeded NHEK cells from donor 1 on day 0 and treated them with 200 μM or 300 μM GO for the next 2 days (Supplementary Figure S1a). We determined the GO treatment concentrations on the basis of a TUNEL assay performed on the treated NHEK cells.
WebNov 9, 2024 · NHEKs (newborn/male, Thermo Fisher Scientific, Waltham, MA, USA) were cultured in EpiLife™ medium containing 60 μM calcium supplemented with human keratinocyte growth supplement (both from Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% CO 2. The medium was changed every alternate day until the … WebEpiLife greatly extends the in vitro lifespan of human epidermal keratinocytes compared to other serum-free media systems. This basal media is animal origin free and contains …
WebOn the third day, the system was exposed to an air–liquid interface by removing the medium in the upper compartment of the scaffold. In the microplate compartment, the medium was replaced by 4 mL of Epilife medium supplemented with 1.5 mM calcium, 10 ng/mL KGF and vitamin C (100 µg/mL), no longer submersing the upper compartment of …
WebEpiLifeはヒト表皮ケラチン生成細胞およびヒト角膜上皮細胞の長期無血清培養用に塩化カルシウム60µMで調製された滅菌液体培地です。この基本培地は、使用前に適切な成長 … frt collins roadsWebEpiLife® Medium Calcium-Free and EpiLife® Medium Calcium- and Phenol Red-Free are sterile, liquid tissue culture media intended for use as one component in a complete … frtcxWebDescription. A sterile, liquid medium prepared with 60μM calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes … gibson decals headstockWebEpiLife is a sterile, liquid medium prepared with 60 µM calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. … frt colling hikingWebEpiLife® Medium. Without calcium. A sterile, liquid medium prepared without calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes and … frtc sgWebA sterile, liquid medium prepared with 60 M calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. This basal … frtcvWebFeb 28, 2024 · NHEKs were obtained from Invitrogen (Life Technologies) and maintained in EpiLife medium (Life Technologies) supplemented with 60 μM calcium, epidermal growth factors, penicillin, and streptomycin. After a 4-hour (tumor cell lines) or 24-hour (NHEK) incubation with 6-HAP, proliferative activity of cells was colorimetrically determined by ... frt cusip